Antioxidants are substances that can inhibit the oxidation process, so that they have a protective effect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto-Botto leaves (Chromolaena odorata L.). This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods. The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods. The  n-butanol  fraction  of  Botto-Botto  leaves  had  the  highest  antioxidant  activity,  namely  the  strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 μg/ mL and 60.885 μg/mL, respectively. The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction.

Karlina Amir Tahir has completed her Ph.D at the age of 35 years from Universitas Hasanuddin, Makassar, Indonesia. She is a lecturer of Department of Pharmacy, Faculty of Medicine and Health Science, Indonesia. She has over 20 publications that have been cited over 37 times, and her publication h-index is 5.